Seminario impartido por el Prof. Jesús Jiménez Barbero, actual Premio Nacional de Química
Molecular recognition by specific targets is at the heart of the life processes. The interactions between proteins (lectins, enzymes, antibodies) and carbohydrates mediate a broad range of biological activities, from fertilization and tissue maturation to pathological processes. The elucidation of the mechanisms that govern how sugars are accommodated in the binding sites of these receptors is currently a topic of interest. Thus, unravelling the structural and conformational factors and the physicochemical features that rule the interactions of these molecules is of paramount interest. Solution NMR is unique in providing stereochemical and conformational information. Given the inherent flexibility and dynamic properties of sugars, we use NMR as key tool for deducing at atomic resolution molecular recognition processes in which glycans are involved, also assisted by a variety of synthetic, molecular biology, computational and biophysical techniques. This presentation is focused on the application of state-of-the-art NMR methods both from the ligand and receptor’s perspective to study molecular recognition processes between receptors of biomedical interest and glycans. As recent examples, key details of glycan recognition by these receptors will be shown, with special emphasis in the application of novel 13C-based and paramagnetic-NMR methods, including the interactions of the spike protein of SARS CoV-2 with human immune lectins and cell glycans.1-8 References Unione et al., ACS Chem Sci. 2019, 5, Gimeno et al., Opin. Struct. Biol. 2020, 62, 22–30. Lenza et al., Angew Chem Int Ed. 2020, 59, 23763. Moure et al., Angew Chem Int Ed. 2021, 60, 18777–18782. Unione et al., Opin. Struct. Biol. 2021, 68, 9-17. Unione et al., Angew Chem Int Ed, 2022, 61: e202201432 Bertuzzi et al., ACS Chem Sci. 2022, 8, 1415. Lenza et al., JACS Au. 2023, 3, 204.
Fecha del seminario: 10/10/2023 12:00
Lugar del seminario: salón de actos IQF
Ponente del seminario: Prof. Jesús Jiménez Barbero
